Study on repellent effect of basil extract (ocimum basilicum l. Lamiaceae) on aedes aegypti in experimental animals

Thị Minh Thu Nguyễn, Minh Khiết Đoàn

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Abstract

This study was carried out between June and August, 2020 at National Institute of Malariology, Parasitology and Entomology, Hanoi, Vietnam.


Objective: To test repellent effect of basil extract (Ocimum basilicum L. Lamiaceae) on Aedes aegypti mosquitoes in experimental animals.


Methods: Repellent effect of basil extract on Aedes aegypti was assessed according to WHO method in guinea pigs. Total of 900 Aedes aegypti mosquitoes were selected and divided in 9 groups, in which each group had 100 mosquitoes. Three groups were considered as the control ones that were kept respectively in a tunnel with blank nets. Three others were caged in turn at the same tunnel with the net which had been soaked in the basil extract at the concentration of 100 g/100 mL, while the rest were confined in the tunnel in succession with 200 g/100 mL basil-extract-dipped net. Then, the rates of dead mosquitoes and sucking blood inhibition were evaluated after exposing 15 hours to basil-extract nets.


Results: At the concentrations of 100 g/100 mL and 200 g/100 mL, basil extracts revealed average repellent effects on Aedes aegypti with the rates of 62.71% and 76.12%, respectively.

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References

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