Nghiên cứu định chuẩn hóa học và độc tính cấp trên động vật thực nghiệm của chế phẩm giảm cân CHM-WL

Abstract

Objectives: The objectives of this research are to prepare weight loss hard capsules from Amorphophallus konjac, green tea leaf and green coffee bean extracts to quantitatively determine acive compounds in the capsule ingredients, and then to assess the safety of weight loss hard capsules.

Subjects and methods: Amorphophallus konjac, green tea leaf and green coffee bean extracts were prepared following solvent extraction method and then vacuum dryied by a rotary evaporator to dryness. Then, a formula

for weight-loss dry capsules was developed by mixing main ingredients with chosen excipients. The bioactive compounds containing in the ingredient extracts and the capsule were examined using a spectrophotometer and HLC-DAD-QTOF (model X500R, Sciex). The assessment of capsule safety was performed on white mice fed with weight-loss capsules at increasing dose then observed for standard parameters following OECD guide.

Results: Study uses preliminary standards glucomannan, chlorogenic acid and EGCG to quantify its corresponding content of 81,0% KGM in KGM-rich Amorphophallus powder, 48,0% chlorogenic acid in green coffee been extract and 33,1% EGCG in catechin-rich green tea leaf extract. Each weight loss hard capsule CHM-WL composing of 230mg KGM-rich Amorphophallus powder, 145mg catechin-rich green tea leaf extract, 100mg chlorogenic acid-rich green coffee been extract was quantified with three mentionned preliminary standards showed their corresponding contents of 186,3mg KGM; 47,9 mg chlorogenic acid and 48,0 mg EGCG. The product CHM-WL showed no acute toxicity at dose of 10g/kgP.