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Date Published:
25/08/2025
Abstract Views: 15
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Views PDF: 13
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Research Articles
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Nguyen, T. T. H. ., Nguyen, T. M. T., Nguyen, P. D., Do, T. B. T., Do, T. T. X., & Vi, V. B. (2025). In vitro antibacterial effects of Alkaloid-rich dried extract from Areca nuts (Semen Arecae Catechi). Viet Nam Journal of Traditional Medicine and Pharmacy, 63(04), 76-80. https://doi.org/10.60117/vjmap.v63i04.443
In vitro antibacterial effects of Alkaloid-rich dried extract from Areca nuts (Semen Arecae Catechi)
Main Article Content
Abstract
Objective: To test in vitro antibacterial effects of Areca nut dried extract rich in alkaloids.
Subjects and methods: The test was conducted on three bacterial strains comprising Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 11775 by the agar well diffusion method.
Results: Areca nut extract (ANE) at all tested concentrations exhibited remarkable in vitro antibacterial activity against three strains of P. aeruginosa, S. aureus and E. coli with their largest antibacterial ring diameters at 1.0 g/ml being 17.10 ± 0.707, 26.67 ± 0.661 and 25.00 ± 0.90 mm, respectively. The diameter values decreased linearly with the test sample’s concentrations and were lowest at 0.1 g/ml with values of 7.90 ± 0.64, 12.20 ± 1.45 and 15.80 ± 11.33 mm in order. Moreover, ANE had the highest antibacterial activity against S. aureus, significantly higher than E. coli (p<0.05) and P. aeruginosa (p<0.01) at each of the same test concentrations. Also, ANE’s antibacterial effects reached highest when the bacteria were exposed to it for 2 hours at 37oC before being cultured for 24 hours.
Conclusion: ANE showed notable in vitro antibacterial activity against three strains of P. aeruginosa, S. aureus and E. coli at various concentrations of 1.0, 0.5, 0.333, 0.25, 0.20, 0.167, 0.143, 0.125, 0.111 and 0.1 g/ml. The best antibacterial effects were achieved when all bacteria were exposed to ANE for 2 hours at 37oC before being cultured.
Article Details
References
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3. Jam N., Hajimohammadi R., Gharbani P., Mehrizad A. Evaluation of Antibacterial Activity of Aqueous, Ethanolic and Methanolic Extracts of Areca Nut Fruit on Selected Bacteria. BioMed Res. Int, 2021, 2021:6663399. Doi: 10.1155/2021/6663399.
4. M Arifur Rahman et al. Comparative antimicrobial activity of Areca catechu nut extracts using different extracting solvents. Bangladesh Journal of Microbiology, 2014, pp.19-23.
5. Bhandare A.M., Kshirsagar A.D., Vyawahare N.S., Hadambar A.A., Thorve V.S. Potential analgesic, anti-inflammatory and antioxidant activities of hydroalcoholic extract of Areca catechu L. nut. Food Chem. Toxicol, 2010, 48, pp.3412-3417. Doi: 10.1016/j.fct.2010.09.013.
6. Nguyen Thi Thuy Hang. Using the active ingredient arecolin in dried Areca nuts to treat internal parasitic diseases in snakehead fish (Channa striata) at the juvenile stage, Grassroots level project 2020, An Giang University, An Giang.
7. Nguyen Phuong Dung, Vu Thi Phuong Thao, Tran Van Thanh. Some factors affecting the extraction efficiency and total alkaloid content in the extract from areca nuts (Semen Arecae catechi). Vietnam Journal of Traditional Medicine and Pharmacy, No. 1(54), 2024, pp.27-31.
8. Mounyr Balouiri, Moulay Sadiki, Saad Koraichi Ibnsouda. Methods for in vitro evaluating antimicrobial activity: A review. J Pharm Anal, 2015, 6(2), pp.71-79. Doi: 10.1016/j.jpha.2015.11.005.
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