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Date Published: 10/11/2021
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DOI: https://doi.org/10.60117/vjmap.v1iđặc%20biệt%2020/11.190
Issue
Vol. 1 No. đặc biệt 20/11 (2021)
Section
Research Articles
How to Cite
Nguyễn, T. N., Đậu, X. C., Đào, T. A., & Nguyễn, H. N. (2021). Deploying the model of knee osteoarthritis in wistar rats. Viet Nam Journal of Traditional Medicine and Pharmacy, 1(đặc biệt 20/11), 121-126. https://doi.org/10.60117/vjmap.v1iđặc biệt 20/11.190

Deploying the model of knee osteoarthritis in wistar rats

Thị Ngọc Nguyễn, Xuân Cảnh Đậu, Thế Anh Đào, Hoàng Ngân Nguyễn

Main Article Content

Abstract

Objective: Knee osteoarthritis model was induced in rats with synovial injection monosodium iodoacetate (MIA).


Subjects and methods: 9-week-old wistar rats were injected with 1.0mg/30µL MIA into kneejoint to cause knee osteoarthritis. The control group was injected with physiological saline. Evaluate the change in the weight bearing with a specialized measuring device (Incapacitance tester); serum PGE2 and proinflammatory cytokines TNF-α, IL-1β, and IL-6; and histopathology of the knee.


Results: Compared with the control group, rats in the MIA injection group had a reduction in the weight bearing (p < 0.001); serum concentrations of PGE2, TNF-α, IL-1β, and serum IL-6 were increased (p < 0.001); knee-joint histopathology showed clear osteoarthritis with manifestations: the knee joint has thick synovial membrane, infected with many inflammatory cells including lymphocytes and macrophages; in some places the synovial membrane has fibrotic necrosis; congestive blood capillaries; cartilage tissue is degenerative and hyalinic.


Conclusion: The model of knee osteoarthritis has been successfully deployed in wistar rats by injecting MIA (monosodium iodoacetate) 1.0mg / 30µL.

Article Details

Keywords

Knee osteoarthritis, monosodium iodoacetate, rats.

References

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