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Ngày xuất bản: 26/09/2022
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DOI: https://doi.org/10.60117/vjmap.v46i5.55
Số xuất bản
Tập 46 Số 5 (2022)
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Do, T. M., Pham, T. P., Tran, T. T. H., & Pham, Q. B. (2022). Multi-response optimization of an extraction procedure of rosmarinic acid from Prunella vulgaris L. and antioxidants using. Tạp Chí Y Dược cổ truyền Việt Nam, 46(5), 4-13. https://doi.org/10.60117/vjmap.v46i5.55

Multi-response optimization of an extraction procedure of rosmarinic acid from Prunella vulgaris L. and antioxidants using

Do Thi Mai1,, Pham Thuy Phuong1, Tran Thi Thu Hien1, Pham Quoc Binh1
1 Viet nam University of Traditional Medicine

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Tóm tắt

Prunella vulgaris L. (PV) have been reported to have a variety of important biological activities. A process for rosmarinic acid (RA) extraction - antioxidants from PV was performed to obtain the highest extraction yield, strongest antioxidant activity and optimized by a multi-response optimization process. A three-level three-factor Box- Behnken design (BBD) was performed as response surface methodology (RSM) with desirability function (D) to attain the optimal extraction parameters. The rosmarinic acid extraction rate was determined by UV-Vis method with linear line Y = 2.1033x + 0.1108, R2 = 0.9966. The DPPH


and ABTS●+ scavenging percentage was used to represent the antioxidant ability. The maximum D value of 0.251, along with the maximum yield (6.025%), %RA (0.115%), scavenging percentage DPPH (IC50) (84.975 µg/ml) and ABTS


(TEAC) (0.207) were achieved after an ethanol


concentration of 60o, an extraction time of 60 min, using an extraction temperature of 50°C.

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Tài liệu tham khảo

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