Thanh bên bài viết

PDF
Ngày xuất bản: 26/09/2022
Số lượt xem tóm tắt: 38
Số lượt xem PDF: 32
DOI: https://doi.org/10.60117/vjmap.v46i5.55
Số xuất bản
Tập 46 Số 5 (2022)
Chuyên mục
Bài nghiên cứu
Trích dẫn bài báo
Do, T. M., Pham, T. P., Tran, T. T. H., & Pham, Q. B. (2022). Multi-response optimization of an extraction procedure of rosmarinic acid from Prunella vulgaris L. and antioxidants using. Tạp Chí Y Dược cổ truyền Việt Nam, 46(5), 4-13. https://doi.org/10.60117/vjmap.v46i5.55

Multi-response optimization of an extraction procedure of rosmarinic acid from Prunella vulgaris L. and antioxidants using

Do Thi Mai1,, Pham Thuy Phuong1, Tran Thi Thu Hien1, Pham Quoc Binh1
1 Viet nam University of Traditional Medicine

Nội dung chính của bài viết

Tóm tắt

Prunella vulgaris L. (PV) have been reported to have a variety of important biological activities. A process for rosmarinic acid (RA) extraction - antioxidants from PV was performed to obtain the highest extraction yield, strongest antioxidant activity and optimized by a multi-response optimization process. A three-level three-factor Box- Behnken design (BBD) was performed as response surface methodology (RSM) with desirability function (D) to attain the optimal extraction parameters. The rosmarinic acid extraction rate was determined by UV-Vis method with linear line Y = 2.1033x + 0.1108, R2 = 0.9966. The DPPH


and ABTS●+ scavenging percentage was used to represent the antioxidant ability. The maximum D value of 0.251, along with the maximum yield (6.025%), %RA (0.115%), scavenging percentage DPPH (IC50) (84.975 µg/ml) and ABTS


(TEAC) (0.207) were achieved after an ethanol


concentration of 60o, an extraction time of 60 min, using an extraction temperature of 50°C.

Chi tiết bài viết

Tài liệu tham khảo

1. Hong-Xi Xu, et al. (1999), “Isolation and characterization of an anti-HSV polysaccharide from Prunella vulgaris”, Antiviral research. 44(1), pp. 43-54.
2. Su-Juan Wang, et al. (2019), “Prunella vulgaris: A Comprehensive Review of Chemical Constituents, Pharmacological Effects and Clinical Applications”, Current pharmaceutical design. 25(3), pp. 359-369.
3. Shi Yong Ryu, et al. (2000), “Anti-allergic and anti-inflammatory triterpenes from the herb of Prunella vulgaris”, Planta medica. 66(04), pp. 358-360.
4. Dejian Huang, Boxin Ou, Ronald L Prior (2005), “The chemistry behind antioXidant capacity assays”, Journal of agricultural and food chemistry. 53(6), pp. 1841-1856.
5. Soraya Rodríguez-Rojo, et al. (2012), “Assisted extraction of rosemary antioXidants with green solvents”, Journal of Food Engineering. 109(1), pp. 98-103.
6. N Mulinacci, et al. (2011), “Storage method, drying processes and extraction procedures strongly affect the phenolic fraction of rosemary leaves: an HPLC/DAD/MS study”, Talanta. 85(1), pp. 167-176.
7. Miguel Herrero, et al. (2010), “Supercritical fluid extraction: Recent advances and applications”,
Journal of Chromatography a. 1217(16), pp. 2495-2511.
8. Kashif Ghafoor, et al. (2009), “Optimization of ultrasound-assisted extraction of phenolic compounds, antioXidants, and anthocyanins from grape (Vitis vinifera) seeds”, Journal of agricultural and food chemistry. 57(11), pp. 4988-4994.
9. Amma G Adomako-Bonsu, et al. (2017), “AntioXidant activity of rosmarinic acid and its principal metabolites in chemical and cellular systems: Importance of physico-chemical characteristics”, Toxicology in vitro. 40, pp. 248-255.