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Date Published: 26/09/2022
Abstract Views: 61
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DOI: https://doi.org/10.60117/vjmap.v46i5.55
Issue
Vol. 46 No. 5 (2022)
Section
Research Articles
How to Cite
Do, T. M., Pham, T. P., Tran, T. T. H., & Pham, Q. B. (2022). Multi-response optimization of an extraction procedure of rosmarinic acid from Prunella vulgaris L. and antioxidants using. Viet Nam Journal of Traditional Medicine and Pharmacy, 46(5), 4-13. https://doi.org/10.60117/vjmap.v46i5.55

Multi-response optimization of an extraction procedure of rosmarinic acid from Prunella vulgaris L. and antioxidants using

Thi Mai Do, Thuy Phuong Pham, Thi Thu Hien Tran, Quoc Binh Pham

Main Article Content

Abstract

Prunella vulgaris L. (PV) have been reported to have a variety of important biological activities. A process for rosmarinic acid (RA) extraction - antioxidants from PV was performed to obtain the highest extraction yield, strongest antioxidant activity and optimized by a multi-response optimization process. A three-level three-factor Box- Behnken design (BBD) was performed as response surface methodology (RSM) with desirability function (D) to attain the optimal extraction parameters. The rosmarinic acid extraction rate was determined by UV-Vis method with linear line Y = 2.1033x + 0.1108, R2 = 0.9966. The DPPH


and ABTS●+ scavenging percentage was used to represent the antioxidant ability. The maximum D value of 0.251, along with the maximum yield (6.025%), %RA (0.115%), scavenging percentage DPPH (IC50) (84.975 µg/ml) and ABTS


(TEAC) (0.207) were achieved after an ethanol


concentration of 60o, an extraction time of 60 min, using an extraction temperature of 50°C.

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