Nghiên cứu áp dụng phương pháp tinh chế hệ dung môi cồn muối làm giàu saponin từ ngưu tất

Abstract

The conditions of purification of saponin from Achyranthes bidentata Blume by the aqueous two-phase system of salt alcohol (NH4)₂SO₄ were determined based on the design of response surface nnethodology (RSM). Total saponin according to oleanolic acid quantified by UV-Vis measurement based on Rosenthaler reaction. The results showed that at the optimal condition including EtOH 34.4g, (NH4)₂SO₄ 18g and 42.5g water, saponin concentrated mainly in the top phase of two phases with recovery up to 83.79%. Applying this condition, the original sage with a refined 7.5% saponin content doubled 15.5% (g/g). Meanwhile, the concentration of saponin when applying the classical two-phase method of n-buntanol/H₂O is only about 13.3%. This method has been shown to be highly effective in enriching saponin, easy to scale up, and environmentally friendly solvents.