Đánh giá độc tính cấp và tác dụng chống viêm của bài thuốc viêm họng mạn tính HV trên động vật thực nghiệm

Abstract

Objective: To evaluate the acute toxicity in Swiss mice and chronic anti-inflammatory effects on Wistar rats of the "Viem hong man tinh HV" remedy.

Methods: Acute toxicity in Swiss mice was assessed according to Litchfield – Wilcoxon method [2] and WHO guidelines [9]. Chronic anti-inflammatory effects of the remedy were evaluated on the amian pellet- induced granuloma models of inflammation in rats.

Results: Swiss mice were drank "Viem hong man tinh HV" with 5 doses from 83 g/kg to 249 g/kg body weight, 0.2mL/10g/time x 3 times/day, but no mice died or had any usually within 7 days after taking the drug. On Wistar rats drinking "Viem hong man tinh HV" with doses 11.62 g/kg/day and 23.24 g/kg/day during 6 days reduced the mass of granulomas (p <0.05) compared to the group of diseases without using drugs.

Conclusions: The acute toxicity of "Viem hong man tinh HV" had not been identified the LD50 on mice although they were taken the highest dose of 249g herbal medicines/kg body weight. "Viem hong man tinh HV" with doses of 11.62 g/kg/day and 23.24 g/kg/day had anti-inflammatory effect on the amian pellet-induced granuloma models of inflammation in rats, equivalent to Prednisolon dose 5mg/kg/day.