Nghiên cứu tác dụng xua muỗi của tinh dầu hung quế Ocimum Basilicum l. Lamiaceae trên thực nghiệm

Abstract

This study was conducted between June and August, 2021 at Institute of Natural Products Chemistry and National Institute of Malariology, Parasitology and Entomology, Hanoi, Vietnam.

Objective: To test repellent effect of basil oil (Ocimum basilicum L. Lamiaceae) on Aedes aegypti, Anopheles minimus and Culex tritaeniorhynchus mosquitoes in experimental animals..

Methods: Repellent effect of basil oil on three different mosquitoe species were assessed according to WHO method in Guinea pigs. Total of 900 mosquitoes of each species were selected and divided in 9 groups, in which each group had 100 mosquitoes. Three groups were considered as the control ones that were kept respectively in a tunnel with blank nets. Three others were caged in turn at the same tunnel with the net which had been soaked in the pure basil oil while the rest were confined in the tunnel in succession with the net dipped into the combination of basil oil – ethanol 70⁰ (7:3). Then, the rates of dead mosquitoes and sucking blood inhibition were evaluated after exposing 15 hours to basil oil-sample nets.

Results: The pure basil oil and the combination of basil oil - ethanol 70⁰ (7:3) revealed high repellent effects on three tested mosquitoe species with the average repellent rates of 99.99% and 99,98% on Aedes aegypti, 99.33% and 99.35% on Anopheles minimus, 99.96% and 99.90% on Culex tritaeniorhynchus, respectively.