Xây dựng phương pháp định lượng hợp chất 3-O-Caffeoyquinic acid trong hạt cà phê xanh ở Việt Nam bằng phương pháp HPLC-UV

Minh Hà Lê, Thi Thanh Huyền Đỗ, Thị Phương Ngọ, Anh Tuấn Phương, Văn Nhân Lê, Văn Thanh Trần

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Abstract

3-O-caffeoyquinic acid (3-CQA) is the main chlorogenic acid found in coffee beans, which possesses a range of valuable bioactivities. In present paper, we report quantitative method of 3-CQA in green coffee beans of Vietnam using HPLC technique. The developed method was showed high repeatability, high accuracy and good sensitivity (LOD and LOQ of 3-CQA were 0.027 and 0.091 ppm, respectively). Applying this method for quantitative assay of 3-CQA in some types of green coffe beans collected in Vietnam (Gia Lai, Lam Dong provinces), the content of 3-CQA was determined from 2,01 – 2,90 % compared to dry sample weight.

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References

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