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Quantifying total alcaloid and evaluating the no production inhibitory effect in raw 264.7 cells of pilea boniana gagnep. harvested in Cao Bang
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Abstract
Objectives: To quantify total alcaloids and evaluate anti-inflammatory activity via the NO production inhibitors on RAW 264.7 cells of Pilea boniana Gagnep.
Subjects and methods: Quantifying total alcaloid content in the Pilea boniana Gagnep. by weighing method and evaluating anti-inflammatory activity through the NO production inhibitor model on RAW 264.7 cells are stimulated by lipopolisaccharid.
Results: The total alcaloid was quantified by the weighing method, according to which the alcaloid content obtained was 1.04 ± 2.32%. The anti-inflammatory activity of the whole and alcaloid extract was evaluated through the ability to inhibit NO production in RAW 264.7 cells showing the IC50 value ranging from 1.27 to 2.68 µg/ml.
Conclusions: The total alcaloid content is relatively high and the production inhibitors in RAW 264.7 cells of the sample is quite strong, especially in total alcaloid dry extract with IC50 of 1.27 ± 0.09µg/ml.
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Keywords
Pilea boniana Gagnep., alcaloid, anti-inflammatory.
References
2. Nguyễn Khắc Khôi và Dương Thị Hoàn. Đặc điểm và phân bố của các loài cây thuốc họ gai (Urticaveae) ở Việt Nam, Hội nghị khoa học toàn quốc về sinh thái và tài nguyên sinh vật lần thứ 2, 2007.
3. Phạm Thanh Kỳ. Dược liệu học, Nhà xuất bản Y học, Hà Nội, 2007, tập 2.
4. Bộ Y tế. Dược điển Việt Nam 5, Hà Nội, 2018, tập 2.
5. Cheenpracha S., Park E.-J., Rostama B. et al. Inhibition of Nitric Oxide (NO) Production in Lipopolysaccharide (LPS)-Activated Murine Macrophage RAW 264.7 Cells by the Norsesterterpene Peroxide, Epimuqubilin A. Marine Drugs, 2010, 8(3), pp.429–437.
6. AOAC International. AOAC official methods of analysis, Appendix F: Guidelines for Standard Method Performance Requirements., 2016.
7. Facchin B.M., Dos Reis G.O., Vieira G.N. et al. Inflammatory biomarkers on an LPS-induced RAW 264.7 cell model: a systematic review and meta-analysis. Inflammation Research, 2022, 71(7–8), pp.741–758.
8. Merly L. và Smith S.L. Murine RAW 264.7 cell line as an immune target: are we missing something? Immunopharmacology and Immunotoxicology, 2017, 39(2), pp.55–58.