EVALUATING THE CYTOTOXIC EFFECT OF THE ROOT EXTRACT OF SYMPLOCOS PANICULATA (THUNB.) MIQ.
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Abstract
Objective: To develop an extraction procedure for the roots of Symplocos paniculata and to evaluate the cytotoxic effect of the extract on two cell lines: A549 (lung cancer) and MCF-7 (breast cancer).
Subjects and methods: Various factors affecting the extraction process of the total extract from S.paniculata roots were investigated. Liquid-liquid extraction was performed using solvents of increasing polarity to obtain different extract fractions. The cytotoxic effects of the extracts on A549 and MCF-7 cell lines were evaluated using the Sulforhodamine B (SRB) cell cytotoxicity assay.
Results: The optimized extraction process yielded a total saponin content, calculated as oleanolic acid equivalent, of 21,11% on average, with an average extraction yield of 17,02%. Both the total extract and the fractions exhibited cytotoxic activity against the tested cancer cell lines, with IC₅₀ values ranging from 7,76 to 14,20 µg/mL. The n-hexane fraction (SPR-H) showed the strongest cytotoxic activity.
Conclusion: An optimized extraction process for the crude extract from S.paniculata roots was successfully developed. The tested extracts, particularly the n-hexane fraction (SPR-H), demonstrated significant cytotoxic activity against both A549 and MCF-7 cancer cell lines.
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References
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