ESTABLISHING A RAT MODEL OF CHRONIC KIDNEY DISEASE VIA FIVE-SIXTHS NEPHRECTOMY
Main Article Content
Abstract
Objective: To develop a rat model of chronic kidney disease through surgical removal of five-sixths of total renal mass, serving as a foundation for future pharmacological and therapeutic studies.
Subjects and methods: Experimental study on white rats, randomly divided into 10 rats/group, including control group with surgery without kidney removal and given distilled water, model group with surgery to remove 5/6 of the rats' kidneys and given distilled water.
Results: After 2 weeks of surgery to remove 5/6 of the rats's kidneys, in the model group, the concentrations of urea, creatinine, 24-hour urine volume and 24-hour proteinuria of the rats increased compared to before surgery. Histological images of the rats kidneys showed interstitial expansion, glomerular hypertrophy, and tubular dilation. The survival rate of rats was 100%.
Conclusion: The 5/6 nephrectomy model successfully reproduced key clinical and pathological features of CKD with a high survival rate. This stable and reproducible model provides a valuable experimental basis for studying the pathophysiological mechanisms of CKD and for evaluating potential pharmacological or therapeutic interventions in future research.
Article Details
References
2. Do Gia Tuyen. Chronic kidney disease and chronic renal failure definition and diagnosis. Internal medicine, volume 1, Medical Publishing House, Hanoi, 2023, p.566.
3. R Lozano MD et al. Global and regional mortality from 235 causes of death for 20 age groups in 1990 and 2010: A systematic analysis for the Global Burden of Disease Study 2010. The lancet, 2012, 380, 2113.
4. Lu JR, Han HY, Chen J., et al. Protective Effects of Bu-Shen-Huo-Xue Formula against 5/6 Nephrectomy-Induced Chronic Renal Failure in Rats. Evid. Based Complement. Altern. Med, 2014, 589846. 10.1155/2014/589846.
5. Nakagawa T., Tashiro I., Fujimoto M., et al. Keishibukuryogan reduces renal injury in the early stage of renal failure in the remnant kidney model. Evidence-based complementary and alternative medicine: eCAM, 2011, 914249.
6. Hayashi K, Shimokawa T, Yamagata M, Yoneda K. Inhibition of α 2 - adrenoceptor is renoprotective in 5/6 nephrectomy-induced chronic kidney injury rats. J Pharmacol Sci, 2021 Jan, 145(1), pp.79-87, doi: 10.1016/j.jphs.2020.11.001, Epub 2020 Nov 17, PMID: 33357783.