Khảo sát một số yếu tố ảnh hưởng quá trình chiết xuất lá cây Đơn lá đỏ (Excoecaria cochinchinensis Lour.)

Abstract

Objective: To investigate and develop an extraction process from Excoecaria cochinchinensis Lour. leaves to obtain the highest flavonoid content for application in traditional medicine.

Subjects and methods: The leaves of Excoecaria cochinchinensis were subjected to single-variable experiments to evaluate the effects of solvent type, extraction temperature, material-to-solvent ratio, number of extraction cycles, and particle size of the material on the total flavonoid content. The total flavonoid content was quantified using UV-Vis spectroscopy with the aluminum chloride (AlCl₃) colorimetric method, employing kaempferol as the standard. The response surface methodology, based on the Box-Behnken experimental design with three factors and three levels, was applied to determine the optimal extraction conditions.

Results: The optimal extraction process was determined under the following conditions: 70% ethanol as the solvent, a material-to-solvent ratio of 1/14, an extraction temperature of 80°C, an extraction time of 3 hours, and three extraction cycles. Under these conditions, the extract yielded a flavonoid content of 1,02%.

Conclusion: The extraction process from Excoecaria cochinchinensis has been optimized, yielding a high flavonoid content and laying the foundation for pharmaceutical applications.