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Extracting and evaluating the in vitro α-glucosidase inhibitory activity of the Symplocos paniculata (thunb.) Miq. Leaf extract
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Abstract
Objectives: To develop an extraction procedure for leaves of Symplocos paniculata and evaluate the α-glucosidase inhibitory activity of resulting extracts.
Subjects and methods: The extraction conditions were investigated to obtain crude extracts from S. paniculata leaves. Liquid-liquid extraction was performed using solvents of increasing polarity to obtain fractions. All the extracts and fractions were then evaluated for their α-glucosidase inhibitory activities.
Results: The established procedure for S. paniculata leaves resulted in an average yield of 18.851% and an average flavonoid content of 39.854% (quercetin equivalent). The extracts exhibited strong α-glucosidase inhibitory activities, with IC₅₀ values ranging from 13.98–58.99 µg/mL compared to that of the positive control Acarbose 122.31± 9.69 µg/mL.
Conclusion: The extraction process of total extract and fractionated extract from the leaves of S. paniculata achieved a high stability. The inhibitory effect of α-glucosidase enzyme of the extracts from the leaves of S. paniculata was significantly more potent than that of the Acarbose control.
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References
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