Hoạt tính gây độc tế bào ung thư từ cao chiết Ethanol lá cây Cà hai lá (SOLANUM DIPHYLLUM L.)

Abstract

Objective: To evaluate the cytotoxic activity against cancer cells of the ethanol extract of Solanum diphyllum L. leaves.

Subjects and methods: Ethanol 40% and ethanol 70% extracts of leaves were used to conduct the tests. Cytotoxic activity was performed based on the MTT (3 - (4,5 - dimethylthiazol - 2 - yl) - 2,5 – diphenyltetrazolium bromide) method.

Results: The percentage of inhibition of cancer cell lines was proportional to the tested concentration. The 40% ethanol extract exhibited IC₅₀ values of 158.12 µg/mL, 136 µg/mL, 128.02 µg/mL, and 155.12 µg/mL against KB, HepG2, A549, and MCF - 7 cells, respectively. The 70% ethanol extract showed IC₅₀ values of 98.86 µg/mL, 62.7 µg/mL, 50 µg/mL, and 117.89 µg/mL against KB, HepG2, A549, and MCF - 7 cells, respectively.

Conclusion: Ethanol 40% and ethanol 70%  have cytotoxic activities on KB, Hep G2, A549 and MCF - 7 cells. Cytotoxic activity on A549, Hep G2 is stronger than on KB, MCF - 7 cells. 70% ethanol extract has stronger cancer cell inhibitory activity than 40% ethanol extract.