Nghiên cứu ảnh hưởng của dịch chiết húng quế Ocimum basilicum L. Lamiaceae đến chức năng thận thỏ

Abstract

This study was carried out between May and July 2021 at National Institute of Malariology, Parasitology and Entomology, and Hanoi University of Medicine, Vietnam.

Objective: To assess the effects of basil extract (Ocimum basilicum L. Lamiaceae) on experimental rabbits’ renal functions.

Methods: The Vietnam Ministry of Health’s and OECD’s guidelines for sub-chronic toxicity testing were applied. The aqua basil extract was treated orally in two different groups with the dose regimens of 0.6 and 1.8 g/kg/day x 28 consecutive days, respectively. A control group treated orally with distilled water was also tested simultaneously. Two milliliters of blood were pulled out from each rabbit’s ear vein on day 0 (before testing), day 14 (the middle of testing) and day 29 (after just stopping taking basil extract) in order to test for creatinine parameter. Less than half of rabbits from each group were operated on day 29 and the others were finished operations on day 43 (after 15 days taking no basil extract) for general kidney observations and microbody structures of renal cells.

Results: The concentrations of creatinine in rabbits’ serum samples of the treated groups were insignificantly different from that of the control group on the same testing days; furthermore, there were unremarkable changes of this parameter between days 14 or 29 and day 0 (the p values > 0.05). Moreover, macroscopic kidney parenchyma of all rabbits on days 29 and 43 were in normal, soft, smooth, homogeneous, reddish-brown, and non-congestive textures.

Microscopically, the rabbits of the control and treated groups had normal renal tubules. Nevertheless, some of them had mild glomerular congestions with the ratios of 2/5 (40.0%), 2/5 (40.0%) and 3/5 (60.0%) on day 29 and 3/6 (50.0%), 2/6 (33.33%) and 3/6 (50.0%) on day 43, respectively. In general, the aqua basil extract did not affect experimental rabbits’ renal functions.