Một số yếu tố ảnh hưởng đến hiệu suất chiết và hàm lượng hoạt chất trong cao của nấm Cordyceps militaris

Abstract

Aims: To choose solvent concentration (ethanol) and temperature for extraction to ensure highest efficiency and contents of active ingredients in the extract from Cordyceps militaris mushroom.

Methods: Extraction of Cordyceps millitaris with ethanol of different concentrations and at different temperatures; Adenosine and Cordycepin in the extracs were assayed by by high performance liquid chromatography (HPLC).

Results: Extraction of cordyceps militaris mushroom by ethanol solvent (EtOH) at concentrations: 300; 600 and 900 gave the extraction efficiency: 54.95%; 42.23%; and 36.18% respectively; adenosine content: 1.2 mg/g;

0.24 mg/g and unidentified, respectively; average content of cordycepin (mg/g): 1.88 mg/g; 1.69 mg/g and

1.73 mg/g, respectively. Extraction in 300 EtOH at different temperature conditions: room temperature (25oC); 40oC; 60oC and 80oC give extraction efficiency (%) respectively: 53,4 %; 54,95 %; 52,35 %; 50,26%; average adenosine content: 1.13 mg/g; 1.2 mg/g; 0.78 mg/g; 0.65 mg/g, respectively; content of cordycepin: 1.81 mg/g;

1.88 mg/g; 1.91 mg/g; 2.1 mg/g, respectively.

Conclusion: The extraction efficiency and content of active ingredients in Cordycept millitaris extracs were influenced by solvent concentration (ethanol) and temperature. Extraction with 300 ethanol with ultrasonic vibration at 40oC is the optimal extraction condition to obtain the highest extraction efficiency and highest content of active ingredients.